Cold Preservation with a Hyperbranched Polyglycerol-Based Solution Improves Functional Recovery of Kidney Transplants in a Pig Model
Caigan Du1, Shadan Li1,2, Qiunong Guan1, Irina Cafeeva3, Donald E Brooks3, Jayachandran N Kizhakkedathu3,4, Christopher YC Nguan1.
1Department of Urologic Sciences, University of British Columbia, Vancouver, BC, Canada; 2Department of Urology, Chengdu Military General Hospital, Chengdu, P.R. China; 3Centre for Blood Research, and the Department of Pathology and Laboratory Medicine, University of British Columbia, Vancouver, BC, Canada; 4Department of Chemistry, University of British Columbia, Vancouver, BC, Canada
Background: Organ preservation solutions are used to limit donor organ injury during cold preservation (perfusion and storage) in organ transplantation, and hyperbranched polyglycerol (HPG) is a promising colloid for the solutions to improve donor organ protection. This study was to evaluate the outcomes of kidney transplants after cold preservation with a HPG-based organ preservation solution in a human-like model
Materials and Methods: The orthotopic kidney autotransplantation was performed in farm pigs (Sus scrofa domesticus, weighing 35–45 kg) under general anesthesia. The left kidney was perfused with and stored in either cold HPG-based organ preservation solution or cold UW solution, followed by autologous transplantation to the right side after right nephrectomy. The survival and function of transplanted kidneys were determined by using their primary outcome-urine production.
Results: The cold ischemia time in both groups (HPG versus UW) was similar (both median 7.625 hours, P = 0.9376, t-test, n=6). Within 7 days after transplantation, the survival of transplanted kidneys between these two groups was not significantly different (P = 0.09193, Log-rank test). The urine output from the first day post-transplantation in HPG group was 1593.33 ± 887.39 mL, which was significantly higher than 310.83 ± 419.48 mL in UW group (P = 0.0095, t-test, n = 6), and this difference was consistent when the urine production between the HPG and the UW groups was compared during the entire period of 7 days (P = 0.0002, two-way ANOVA, n = 6).
Conclusion: Our preliminary data show that as compared to the UW solution, the cold preservation of donor kidneys with the HPG solution has less negative impact on the immediate functional recovery of kidney transplants after transplantation in the pig model, which may suggest that the HPG solution likely improve donor kidney preservation, resulting in better outcomes for kidney transplants.
This study was funded by the Canadian Institutes of Health Research and the Natural Sciences and Engineering Research Council of Canada . Dr. Shadan Li received scholarship from the China Scholarship Council and funding support from General Hospital of Chengdu Military Command (Grant No.: 2016KC28), Science and Technology Department of Sichuan Province (Grant No.: 2017HH0073) and the Center of Donation After Cardiac Death, Center of Medical Science of Chengdu Military Command.
