Xenotransplantation Posters

Tuesday July 03, 2018 from 16:30 to 17:30

Room: Hall 10 - Exhibition

P.536 Composite tissue xenopreservation

Fatih Zor, United States

Dept. of Surgery
Wake Forest Institute for Regenerative Medicine

Abstract

Composite Tissue Xenopreservation

Fatih Zor1, Rezarta Kapaj4, Yalcin Kulahci2, Yildirim Karslioglu3.

1Dept. of Surgery, Wake Forest Institute for Regenerative Medicine, Winston Salem, NC, United States; 2University of Pittsburgh, Pittsburgh, PA, United States; 3Private Practice, Ankara, Turkey; 4Private Practice, Tirana, Albania

The need for preserving composite tissues and using them when needed is a clinical necessity in modern plastic surgery practice. In certain instances, amputate needs to be preserved and used at a future date, as in the amputation patient whose clinical situation will not allow a long surgical procedure.  An appropriate preservation method for composite tissues has not been described up to date. In this study, preservation of composite tissue blocks via xenotransplantation (namely, xenopreservation) is described and short-term results are evaluated.
Two concordant species; Sprague Dawley Rats (n=6) and mice (n=6) were used. The groin flap of the rat was used as a xenotransplant and the vessels on the neck of the Mouse used as recipient vessels. The groin flap of the rat was transported to the neck area of the carrier mouse and microanastomoses were performed between the femoral pedicle of the flap and common carotid artery and external jugular vein of the mouse. Immunosuppression was administered in order to prevent rejection. After a 7 days period of preservation on the site, xenotransplanted groin flap is re-harvested, samples were collected from the skin and vascular structures and the flap was carried to the donor’s opposite groin area. Anastomoses were performed between the flaps pedicle and the femoral artery and vein. The flap was monitored daily. Fifteen days after the second surgical procedure the rats were euthanized and samples were collected. All the samples were evaluated by Haematoxylene-Eosine stain.
All xenopreserved groin flaps survived indefinitely. Tissue evaluation indicated prominent inflammation in carrier mouse, but these changes were partially when the xenopreserved tissue is transplanted to the donor rat.
In this study a method for composite tissue preservation and a basic model for further investigation has been developed in this study. Additional studies are needed in order to find diverse strategies to modulate the tissue changes.



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